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M9480873.TXT
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1994-09-05
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Document 0873
DOCN M9480873
TI Isolation of fastidious mycobacteria from patients with AIDS.
DT 9410
AU Yajko DM; Clancy MN; Goodman C; Hadley WK; Univ. of California, San
Francisco General Hospital.
SO Abstr Gen Meet Am Soc Microbiol. 1994;94:180 (abstract no. U-45). Unique
Identifier : AIDSLINE ASM94/94313072
AB During a recent 14 month period (6/92-8/93) slow-growing fastidious
mycobacteria were recovered at San Francisco General Hospital from 22
specimens submitted from 10 patients with AIDS. Twenty of the isolates
(from 9 patients) were recovered from blood and two isolates (from a
single patient) were recovered from AFB smear positive sputum specimens.
Nineteen of the blood isolates (from 8 patients) grew in Bactec 12B
broth with a median time to detection of 42 days (range 14-54 days).
Three isolates (a blood isolate from one patient and the two sputum
isolates from another patient) did not grow in Bactec 12B broth but were
seen on Middlebrook 7H11 agar after 49-54 days of incubation. None of
the isolates grew on Lowenstein-Jensen medium but in most cases colonies
were seen on the primary non-selective 7H11 agar plate or on the first
sub-culture from 12B broth to 7H11 agar. Further sub-culture to solid
media (7H11 agar, 7H11 with 5% human blood, 7H10 with mycobactin-J,
chocolate agar) was often unsuccessful in our laboratory but growth
usually occurred by sub-culturing to 7H9 broth. Isolates from 4 of the
patients have been identified by the California State Dept of Health as
M. genavense. Identification of the isolates from the remaining patients
is still in progress. The results of this study indicate that isolation
of fastidious mycobacteria from our patient population is not uncommon
and that mycobacterial blood cultures from patients with AIDS should be
held for > 6 weeks since one-half of the fastidious isolates were
recovered after 42 days of incubation.
DE AIDS-Related Opportunistic Infections/BLOOD/*MICROBIOLOGY Human
Mycobacterium/CLASSIFICATION/GROWTH & DEVELOPMENT/*ISOLATION & PURIF
Sputum/MICROBIOLOGY Tuberculosis/BLOOD/COMPLICATIONS/*MICROBIOLOGY
MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).